I suppose, like most photographers, I have a “photographic bucket-list”, and documenting the life-cycle of the much maligned Mosquito has always been high on that list. Like many of my projects, this turned out to be quite the undertaking, filled with many challenging and unique problems.
Mosquito Photography
Mosquitoes start off as eggs, which hatch into larvae. The larvae, also called wrigglers, feed on algae and micro-organisms in the water. They spend much of their time at the water’s surface sucking oxygen through breathing tubes attached at their tail. After about a week, they turn into pupae.
The pupae do not feed. Like larvae, pupae spend much of their time at the water’s surface breathing through two tubes called trumpets. This stage is very much like a Butterfly’s cocoon. This is where the larvae transform into a Mosquito. If you look closely at the photo below, you can actually see the eye of the mosquito of this pupae which is very close to hatching. 
The technical challenges in these first two stages were mainly created by how tiny my subjects were. I was working at magnifications of 6X to 7X. The curled up pupae is only about the size of this o! Additionally,, photographing through glass and water proved a challenge as well.
Photos of Mosquitos
After a couple of days,, the adult Mosquito has formed inside. At this point the pupae unrolls flat on its back on the water’s surface, and the adult Mosquito extracts itself from the pupal case. Once emerged, it rests for a few minutes on the water’s surface, allowing its body to harden and pumping fluid into its wings. The challenge here is being on-site when this happens. The whole event from unroll to first flight lasts about five minutes! I can tell you I missed it much more than I witnessed it! I had to be all set up and ready to shoot at a moment’s notice. I was lucky to witness three hatches over the course of four days (and nights). The first time I witnessed it, I was unable to shoot due to a dead battery. I can tell you that did not happen again!
Here is a shot of the setup used to create these images. All images were created with the Canon MPE-65 1X to 5X lens. I used three flashes: two for the subject, and the third light above the camera lights the background. The LED light provides enough light for critical focus.
When working at such extreme magnifications It is far easier to fine-tune the focus by moving the camera back and forth rather than using the focusing ring. I find the Cognisys StackShot electric rail an excellent tool for that. I was even able to create one stacked image (see below).
Much to my surprise I was even able to create one focus-stacked image. This image is a composite of five different images created with the help of the Cognisys StackShot. Each of the five images is shot at a different focus point and then combined into one final image in the computer. This technique allows me to get enough depth of field to create this image.
I have to admit… for the last few days I haven’t been able to swat any of the Mosquitoes flying around my house for fear that they may have been one of my models!
Good Luck and Good Light!
Steve